Impact of microbial inoculum storage on dark fermentative H2 production

Complex organic substrates represent an important and relevant feedstock for producing hydrogen by Dark Fermentation (DF). Usually, external microbial inoculum originated from various natural environments is added to seed the DF reactors. However, H2 yields are significantly impacted origin storage conditions as community composition can fluctuate. This study aims determine how type time of impact performances. Biochemical Hydrogen Potential tests were carried out using three (glucose, fraction municipal solid waste, food waste), inocula different origins, (freezing or freeze-drying) duration. As a result, production glucose with differently stored was (positively negatively) inoculum-origin-dependent. For complex substrates, not statistically fresh inocula, offering possibility store inoculum.